There's no way that the Joker will ever not be around in Batman's world. He'll always be there to some capacity, but many of his appearances have led to his death over the years. In fact, the Joker's original appearance ended with his death, but it was later retconned. More recently, the Joker fell to his death during Scott Snyder's run.
One of his most iconic deaths was his suicide in The Dark Knight Returns, which made it look like Batman had broken his neck. There have also been multiple potential deaths during his time in the animated series, from being trapped in an exploding (and crashing) plane to actually being killed by Tim Drake in Batman Beyond.
Yes, he was presumed dead for decades when he was frozen in ice, but that’s just the tip of the, well, you know. Steve Rogers faked his own death during a Hydra attack in the '60s, and nearly ten years later, he actually died when a cosmic entity called Korvac killed all the Avengers. He also got disintegrated by Doctor Doom during Secret Wars, killed by the Infinity Gauntlet, and shot dead by Crossbones and a brainwashed Sharon Carter. Don’t worry, he got better from all of them.
Charles Xavier, a.k.a. Professor X, may be the leader of the X-Men, but they’ve had to get along without him for a fair amount of time. During the early days of the X-Men, he faked his own death to go into hiding and prepare for an alien invasion. In Uncanny X-Men #167, Xavier was killed by the Brood but was later brought back to life in a clone body. After many fake-outs in the following years, he was finally shot dead by Bishop during the Messiah Complex arc. Of course, he eventually came back, only to be killed by a Phoenix-possessed Cyclops.
There’s also his death in X-Men: The Last Stand… but the less that's said about that, the better.
There have been many times when Logan has completely bought the farm. There's even a whole storyline about his enemies trying to kill him after he loses his healing power. It's called Death of Wolverine and, spoiler alert, they succeed. But that's not the only time he's died: in the original Days of Future Past arc, he was vaporized by a sentinel blast, in a “What If?” issue, the Hulk killed him by breaking his spinal cord (yes, apparently that worked here), and a villain named Sublime once even managed to negate Wolvie's healing power, leaving him completely unprotected.
And, of course, when fan-favorite writer Chris Claremont returned to X-Men with a series called X-Men Forever in 2009, one of his first acts was killing off Logan.
Originally a US Marine who died and was resurrected by the Egyptian moon god Khonshu, Moon Knight is no stranger to coming back from the dead. Whenever he dies - and he's died multiple times - Khonshu will just bring him back when he's needed. His most famous death came after he quit the Avengers and died after becoming trapped in a burning building. He actually stayed dead for several years, until he was needed again and brought back for a 1998 miniseries.
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